Project Title :
Identification and transcriptional profiling of ethylene responsive factors and downstream signalling transducers in the traditional rice cultivars grown in the back water flood-prone zone of the Hirakud dam reservoir.
|Principal Investigator and Affiliation :||Dr Ekamber Kariali|
|Co-Investigator and Affiliation :|
|Funding Agency :||Science & Technology Department, Govt. of Odisha|
|Amount Sanction and Duration :||7.36 Lakhs - August, 2015 to July, 2018|
|Project Proposal :
The farmers on the banks of Hirakud dam reservoir cultivate several traditional rice cultivars that cope with the adverse effects of submergence when rising water level flood the embankments. But they have not been screened for stress resistance. Identification of QTL Sub1 in FR13A, a rice land race grown in the coastal part of Odisha has provided considerable hope for submergence tolerance. This QTL is a cluster of three genes, namely Sub1A, Sub1B and Sub1C and these genes encode ethylene responsive factors, which regulate submergence tolerance. In the ethylene signaling pathway, a family comprising five receptors of the hormone exists in the endoplasmic reticulum. In our recent work, expression profiles of the ethylene receptors (ERS1, ERS2, ETR2, ETR3 and ETR4) and signal transducers (EIN2, EIN3, ERF2, ERR3 and EREBP5) of rice endosperm cells have been identified by RT-PCR and qRT-PCR studies. Normally the transducer proteins induce a cascade of transcription activation events of ethylene responsive genes. The role of ETR2 has been investigated in rice so long, but not the others. In the present study we intend to screen the ethylene receptors and transducers of the submergence tolerant traditional rice cultivars as stated above in comparison to that of FR13A.